Shoot tips and node-bud segments of jujube (Zixiphus jujuba Mill.), derived from subcultured explants, were cultured in vitro on basal Murashige and Skoog medium supplemented with various concentrations of growth regulators and inorganic nutrients.
1. 0.5㎎/ℓ kinetin or 2ip combined with 1.0㎎/ℓ BA increased the plant height, number of nodes, and plant weight.
2. IBA was more effective than IAA or NAA in increasing the plant height and weight, number of nodes, and percent rooting.
3. 1.0㎎/ℓ IBA, when combined with either 1.0㎎/ℓ BA or 0.5 to 1.0㎎/ℓ kinetin, increased the plant height and weight.
4. Addition of adenine sulfate to the basal medium supplemented with 1.0㎎/ℓ IBA and 1.0㎎/ℓ BA (MS＋IBA＋BA) increased the plant height and number of nodes. The optimum adenine sulfate concentrations for shoot tips and node-buds were 40 and 80㎎/ℓ, respectively.
5. Growth responses of shoot tips and node-bud segments to additional sodium phosphate(monobasic, NaHH₂PO₄·H₂O) to MS＋IBA＋BA were different: growth ofshoot tips was enhanced at high concentrations (150 to 250㎎/ℓ) while node-buds at low concentrations(50 to 100㎎/ℓ).
6. Activated charcoal decreased the number of roots and root length. Increase in plant height and fresh weight was observed when the explants were derived from shoot tips and cultured on full strength medium.
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